ABSTRACT
Objective To investigate the effect of resveratrol on proliferation inhibition,cell cycle arrest and apoptosis of Jurkat cell line in acute T lymphoblast leukemia.Methods MTT assay was used to determine the cell vitality.Wright-Giemsa,Hoechest 33258/PI staining and transmission electron microscope technique were used to detect the apoptosis status of Jurkat cells.The cell cycle arrest was analyzed by flow cytometry.(Results Resveratrol) had 64.01% inhibitory rate on the growth of Jurkat cells at 0.2 mmol?L~(-1) and inhibited the growth of Jurkat cells in dose-and time-dependent manner.24 h after treated with resveratrol,the typical features of apoptosis were observed under light and electron microscope in all treatment groups.Some nuclei showed bright blue under fluorescence microscope in the resveratrol-treated Jurkat cells,and the number of cells with bright blue fluorescence increased with time.Nuclei condensation and fragmentation were observed.Cell shrinkage,chromatin condensation,and marginalization were found by Wrigh-Giemsa staining and transmission electron microscope technique.By flow cytometry,62.57% of the cells were arrested at the S phase after exposured to(0.05 mmol?L~(-1)) resveratrol for 48 h,the rate of apoptotic cells to total cells was 12.01% in 0.05 mmol?L~(-1) treatment groups,and that in the control groups was 2.05%. Conclusion Resveratrol can inhibit the proliferation,cause S-phage arrest and induce the apoptosis of Jurkat cells.
ABSTRACT
In this study, the general toxicity tests including acute toxicity test, haemolysis test, MTT assay of Ti-Fe-Mo-Mn-Nb-Zr alloys were carried out. The morphology of these cells was also observed under phase-contrast microscope. By using X-ray photoelectron spectroscopy(XPS), the kind and mol% of element in surface film were studied. The kind and concentration of element in dipping fluid were investigated by ICP atomic emission spectrometry. The results showed the primary component is TiO2 in surface film. The dipping fluid of Ti-Fe-Mo-Mn-Nb-Zr alloys contains Fe 0.2-1.07 mg/l and Mn 0.16-0.5 mg/l; such dental materials are beneficial to health. No cytotoxic effect was disclosed by in vitro and in vivo tests. The level of cytotoxicity was grade 0 and 1; the haemolysis degree was 0.558%-0.642%, i.e. less than 5%. The cells growing in the extract showed normal morphology. These data indicate that Ti-Fe-Mo-Mn-Nb-Zr alloy, as a dental material, has good biocompatibility.